Chemistry · Unit 4 · Properties and structure of organic materials · Structure of organic compounds
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A mixture of three amino acids was separated using paper chromatography with a polar solvent. The $R_f$ value for each amino acid was calculated and recorded in the table below. (a) Identify which amino acid is most soluble in the mobile phase. [1] (b) Explain why amino acids with different $R_f$ values separate from one another during chromatography. [1]
Paper chromatography is used to separate a mixture of amino acids. After development with a suitable solvent, the chromatogram shows three distinct spots with different Rf values. What property of the amino acids is primarily responsible for their separation?
In a thin-layer chromatography (TLC) experiment, a mixture of three amino acids is spotted at the baseline of a silica gel plate and developed using a polar solvent. Which statement correctly explains why the amino acids separate during this process?