Chemistry · Unit 4 · Properties and structure of organic materials · Structure of organic compounds

Analyse data, including paper/TLC chromatograms and electrophoresis to determine the identity of amino acids and retention factors. (Formula: 𝑅F = distance moved by the amino acid distance moved by the solvent)

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Question 1

A student performed thin-layer chromatography (TLC) to identify an unknown amino acid in a sample. The solvent front travelled \(8.5\) cm from the origin, and the amino acid spot travelled \(5.4\) cm from the origin. Analyse the data to identify the unknown amino acid and justify your answer using the retention factor.

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