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Chemistry · Unit 4 · Properties and structure of organic materials · Structure of organic compounds

electrophoresis to separate amino acids*

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Question 1

In gel electrophoresis at pH 7.0, three amino acids (glycine, aspartate, and lysine) are placed at the origin and subjected to an electric field. Which statement correctly describes the direction of migration?

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Question 2

In an electrophoresis experiment, a mixture of three amino acids (alanine, aspartate, and lysine) is placed at the origin on a gel strip at pH 7.0. A potential difference is applied across the strip. Which statement correctly describes the expected movement of these amino acids?

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Question 3

Explain the migration of glycine and aspartic acid during electrophoresis in a buffer solution at pH 6.0.

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Discriminate between saturated and unsaturated organic molecu les.
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Explain how amino acids can be separated and analysed by electrophoresis, including pH of buffer, isoelectric points, and movement of charged ions.
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