Chemistry · Unit 4 · Properties and structure of organic materials · Structure of organic compounds

Explain how amino acids can be separated and analysed by electrophoresis, including pH of buffer, isoelectric points, and movement of charged ions.

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Question 1

A buffer solution at pH 7.6 is used to separate three amino acids by electrophoresis: alanine (isoelectric point 6.0), histidine (isoelectric point 7.6), and lysine (isoelectric point 9.7). Which amino acid will migrate towards the anode (positive electrode)?

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electrophoresis to separate amino acids*

Explain how amino acids can be separated and identified by paper/TLC chromatography, including intermolecular forces/solubility in mobile and stationary phase and retention (RF) values.

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